A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila.
Centrosomes comprise a pair of centrioles surrounded by an amorphous pericentriolar material (PCM).Here, we have performed a microscopy-based genome-wide RNA interference (RNAi) screen in Drosophila cells to identify proteins required for centriole duplication and mitotic PCM recruitment.We analysed 92% of the read more Drosophila genome (13,059 ge